This study describes the development and validation for the simultaneous estimation of desloratadine (DES) and montelukast sodium (MON) by the first-order derivative UV spectroscopy method. The quantification was achieved by the first-order derivative spectroscopy method at 297.20 nm and 339.20 nm over the concentration range of 3-18µg/ml for estimation of desloratadine (r2=0.9993) and 6-36 µg/ml montelukast (r2=0.9999) in a combined tablet formulation. Procedure does not require prior separation of components from the sample. LOD values for DES and MON are found to be 0.08 μg/mL and 0.17 μg/mL, respectively. LOQ values for DES and MON are found to be 0.24 μg/mL and 0.52 μg/mL, respectively. The results of analysis have been validated statistically and recovery studies carried out in the range 80-120% to confirm the accuracy of the proposed method. The relative standard deviation was found to be <2.0%. The present result shows that the proposed method can be successfully used for simultaneous determination of the drug content in marketed formulations.
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